HIV-1 tat, Strain IIIB (Human Immunodeficiency Virus-1) (HRP)

Mab

Blue Ice

Highly Purified

30021010

Molar Ratio:
2-4 peroxidase molecules per molecule of mAb.

Applications:
A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA and Western ELISA may be performed at dilutions of 1:1000 depending on the concentration of tat in the test system. tat capture ELISA may be performed in 1 : 500 dilution range depending on the capture ligand concentration and tat concentration in the test sample.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.