Pregnancy Associated Plasma Protein A (Azide Free) (PAPP-A,Pappalysin 1,PAPA,PAPPA,PAPP-A,PAPPA1,Aspecific BCL2 ARE-binding Protein 2,ASBABP2,Differentially Placenta 1 Expressed Protein,DIPLA1,EC 3.4.24.79,IGF-dependent

Antibodies / Antibodies-Hormones, Steroids

-20°C

Supplied as a liquid in PBS, pH 7.4. No preservative added. Also available with azide. See P6000-80.

Purified human PAPP-A /pro MBP complex.

PAPP A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500kD, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP). In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity. PAPP A is widely used as a marker of some pathologies during pregnancy. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (Trisomy 21).

Applications:
Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Matched Pair:
Capture: Detection:
P6000-82 P6000-80

Hybridoma:
Hybridization of SP 2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.