GAPDH (Glyceraldehyde-3-phosphate dehydrogenase CDABP0047, G3PD, GAPD, Glyceraldehyde-3-phosphate dehydrogenase)

Antibodies / Antibodies-Enzymes, Dehydrogenase

-20°C

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.

Purified, E. coli-derived, recombinant human Glyceraldehyde-3-phosphate dehydrogenase (rhGAPDH; aa 1-150; Accession #P04406).

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a 36-40kD member of the GAPDH family of enzymes. It is a widely expressed heterotetramer that is found in both the nucleus and cytoplasm. Although GAPDH was initially identified as a glycolytic enzyme that converted G3P into 1, 3 diphosphoglycerate, it is now recognized to participate in no less than endocytosis, membrane fusion, vesicular secretory transport, DNA replication and repair, and apoptosis. Human GAPDH is 335 amino acids (aa) in length and contains two NAD binding sites (Asp35 and Asn316) with a catalytic region between aa 151-155. GAPDH contains more than 19 posttranslational modifications, including methylation, deamidation and phosphorylation. One splice variant shows a 10 aa substitution for aa 319-335. Over amino acid 1-150, human GAPDH shares 92% aa identity with mouse GAPDH.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.