Qa-1b (H2 T23, H2 Class I Histocompatibility Antigen D37 alpha Chain, H2 Q1, Histocompatibility 2 T Region Locus 23, MHC Class I Antigen Qa1, MHC ClassIb Qa1, OTTMUSP00000015895, Qa1)

Antibodies / Antibodies-HLA

-20°C

Supplied as a liquid in PBS, 0.05% sodium azide.

Synthetic peptide corresponding to the alpha-2 domain of Qa-1b.

Qa-1, a nonclassical class I histocompatibility molecule encoded by the T23 gene in the T region expressed in mice, predominantly assembles with a single nonameric peptide, Qdm, derived from the signal sequence of certain class Ia molecules. The Qa-1/Qdm complex serves as a ligand for CD94/NKG2 natural killer (NK) receptor(s) express on a major fraction of NK cells and CD8+ immuno-suppressive regulatory T cells. Qa-1, expressing on immuno-regulatory B cells and other antigen presenting cells, serves for antigen-presentation to immuno-regulatory CD8+ T. Moreover, Qa-1 serves for MHC restrictive function because, unlike conventional MHC molecules, it transiently expresses on activated CD4+ T cells. Recently it has also been shown that Qa-1 is ubiquitously expressed in the immune-privileged anterior chamber of the eye. Additionally, it has been suggested that TCR specific, Qa-1-restricted regulatory CD8+ T cells selectively down regulate antigen-activated T cells expressing TCRs of certain affinities. These regulatory CD8+ T cells may control the peripheral TCR repertoire during the course of immune responses to both self and foreign antigens. The 6A8.6F10.1A6 mAb can be used for flow cytometric assay to detect Qa-1b on immuno-regulatory antigen presenting cells and activated splenic T cells on Qa-1b expressing mice (e.g. BALB/c, C57BL/6) and it can also be used for functional assays to block target cell recognition by CTLs specific for Qa-1 restricted antigen presentation and other functional study where Qa-1b associated immuno-regulation may work.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Neutralization and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:10-1:1000
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.