GRP94 Antibody

ArtNr	ABC-ASM10352-BI-100
Hersteller	Abcepta
Menge	Inquiry
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Biotin
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Lieferbar
Primary Accession	P08113
Reactivity	H, M, Rat, B
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP94 Polyclonal Antibody (ASM10352). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP94 Polyclonal Antibody (ASM10352) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Type image 1	ICC/IF
Dilution image 1	1:120
Legend image 2	Western blot analysis of Rat brain cell lysates showing detection of ~ 94-100 kDa GRP94 protein using Rabbit Anti-GRP94 Polyclonal Antibody (ASM10352). Lane 1: MW ladder. Lane 2: Anti-GRP94 (1:250). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GRP94 Polyclonal Antibody (ASM10352) at 1:250 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP antibody at 1:50-1:100 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~ 94-100 kDa. Other Band(s): ~50, ~75 kDa.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP94 Polyclonal Antibody (ASM10352). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP94 Polyclonal Antibody (ASM10352) at 1:120 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Type image 3	ICC/IF
Dilution image 3	1:120
Other Accession	NP_035761.1
Target/Specificity	Detects ~94kDa.
Immunogen	Synthetic peptide corresponding to the sequence near the C-terminus of mouse GRP94
Purification	Peptide Affinity Purified
Reconstitution & Storage	-20ºC

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ABC-ASM10352-BI-100-datasheet.pdf

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Lieferung vsl. bis 02.10.2025

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