Brucella IgM ELISA**

Brucella IgM ELISA**Sample Type: Serum/Plasma# of Samples: 1x96 well
Intended Use: The Inc. Brucella IgM ELISA Kit is intended for the detection of IgM antibody to Brucella in human serum or plasma. Principle of the test: Diluted patient serum (serum diluent contains sorbent to remove Rheumatoid Factor and human IgG interference) is added to wells coated with purified antigen. IgM specific antibody if present binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample. Summary and Explanation of Test: Storage and Stability: 1. Store the kit at 2-8 C. 2. Keep microwells sealed in a dry bag with desiccants. 3. The reagents are stable until expiration of the kit. 4. Do not expose test reagents to heat sun or strong light. Storage and Stability: 1. Store the kit at 2-8 C. 2. Keep microwells sealed in a dry bag with desiccants. 3. The reagents are stable until expiration of the kit. 4. Do not expose test reagents to heat sun or strong light. Limitation of the Test: 1. To enhance sensitivity and specificity of this IgM test provided sample diluent has been formulated to block IgG and Rheumatoid Factor (RF) interferences. Turbidity could be seen after diluting serum with sample diluent. This turbidity is due to the blocking of serum IgG and shows no interference with test results. It can be removed by centrifugation. 2. In specimens with high RF and high autoimmune antibodies the possibility of eliminating the interferences cannot be ruled out entirely. 3. The test results obtained using this kit serve only as an aid to diagnosis and should be interpreted in relation to the patient\' s history physical findings and other diagnostic procedures. 4. Lipemic or hemolyzed samples may cause erroneous results. Sensitivity and

Brucella IgM ELISA**