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Anti-HIF3/HIF3A Antibody Picoband®

Anti-HIF3/HIF3A Antibody Picoband®

ArtNr	BOS-PA2145-FITC
Hersteller	Boster
Menge	100 ug
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Kategorie	Oncology Antibodies Flow Cytometry Western Blot ELISA Primary Antibodies By Organ Lung Cancer Immunostaining
Typ	Antibody Polyclonal
Format	Lyophilized
Applikationen	WB
Specific against	Human (Homo sapiens), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Konjugat/Tag	FITC
Sensitivity	<50 pg/ml
Sequence	Contains 1 bHLH (basic helix-loop-helix) domain.
Citations	1. Hara, S., Hamada, J., Kobayashi, C., Kondo, Y., Imura, N. Expression and characterization of hypoxia-inducible factor (HIF)-3-alpha in human kidney: suppression of HIF-mediated gene expression by HIF-3-alpha. Biochem. Biophys. Res. Commun. 287: 808-813, 2001. 2. Maynard, M. A., Qi, H., Chung, J., Lee, E. H. L., Kondo, Y., Hara, S., Conaway, R. C., Conaway, J. W., Ohh, M. Multiple splice variants of the human HIF-3-alpha locus are targets of the von Hippel-Lindau E3 ubiquitin ligase complex. J. Biol. Chem. 278: 11032-11040, 2003.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Hypoxia-inducible factor 3-alpha,HIF-3-alpha,HIF3-alpha,Basic-helix-loop-helix-PAS protein MOP7,Class E basic helix-loop-helix protein 17,bHLHe17,HIF3-alpha-1,Inhibitory PAS domain protein,IPAS,Member of PAS protein 7,PAS domain-containing protein 7,HIF3A,BHLHE17,MOP7,PASD7
Lieferbar
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	49 kDa
Calculated Molecular weight	72433 MW
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5μg/ml, Rat, Human
Gene Name	HIF3A
Gene Full Name	Hypoxia-inducible factor 3-alpha
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human HIF3, different from the related rat and mouse sequences by five amino acids.
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Neural Stem Cells, Signal Transduction, Stem Cells
Protein Name	Hypoxia-inducible factor 3-alpha
Protein Function	Acts as a transcriptional regulator in adaptive response to low oxygen tension. Acts as a regulator of hypoxia-inducible gene expression (PubMed:11573933, PubMed:16126907, PubMed:19694616, PubMed:20416395, PubMed:21069422). Functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. Plays a role in the development of the cardiorespiratory system. May also be involved in apoptosis (By similarity).
Subcellular Localization	Nucleus. Cytoplasm. Nucleus speckle. Mitochondrion. In the nuclei of all periportal and perivenous hepatocytes. In the distal perivenous zone, detected in the cytoplasm of the hepatocytes. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. Colocalizes with BAD in the cytoplasm. Colocalizes with EPAS1 and HIF1A in the nucleus and speckles (By similarity). Localized in the cytoplasm and nuclei under normoxia, but increased in the nucleus under hypoxic conditions (PubMed:19694616). Colocalized with HIF1A in kidney tumors (PubMed:19694616).
Description	Boster Bio Anti-HIF3/HIF3A Antibody catalog # PA2145. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Expressed in vascular cells (at protein level) (PubMed:21069422). Expressed in kidney (PubMed:11573933, PubMed:19694616). Expressed in lung epithelial cells (PubMed:16775626). Expressed in endothelial cells (venous and arterial cells from umbilical cord and aortic endothelial cells) and in vascular smooth muscle cells (aorta) (PubMed:21069422). Strongly expressed in the heart, placenta, and skeletal muscle, whereas a weak expression profile was found in the lung, liver, and kidney (PubMed:12538644). Expressed weakly in cell renal cell carcinoma (CC-RCC) compared to normal renal cells (PubMed:16126907). Expression is down-regulated in numerous kidney tumor cells compared to non tumor kidney tissues (PubMed:16126907). Isoform 2 is expressed in heart, placenta, lung, liver, skeletal muscle and pancreas and in numerous cancer cell lines (PubMed:20416395). Isoform 3 and isoform 4 are weakly expressed in heart, placenta, lung, liver, skeletal muscle and pancreas (PubMed:20416395). Isoform 4 is expressed in fetal tissues, such as heart, brain, thymus, lung, liver, skeletal kidney and spleen (PubMed:20416395). Isoform 3 is weakly expressed in fetal tissues, such as liver and kidney (PubMed:20416395).
Sequence Similarities	Contains 1 bHLH (basic helix-loop-helix) domain.
Manufacturer - Alias	Hypoxia-inducible factor 3-alpha, HIF-3-alpha, HIF3-alpha, Basic-helix-loop-helix-PAS protein MOP7, Class E basic helix-loop-helix protein 17, bHLHe17, HIF3-alpha-1, Inhibitory PAS domain protein, IPAS, Member of PAS protein 7, PAS domain-containing protein 7, HIF3A, BHLHE17, MOP7, PASD7
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins

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BOS-PA2145-FITC-datasheet.pdf

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