HIF-1 Alpha DNA Binding Activity Assay Kit

Room Temperature

Cell Based Assays

Cell Biolabs’ HiF-1 Alpha DNA Binding Activity Assay Kit Is an Immunoassay developed for rapid detection of activated HiF-1 In any protein sample. Active HiF-1 complex Is captured on a double stranded oligonucleotide containing an HRE that Is attached to the well. HiF-1 Alpha Is then detected with an anti-HiF-1 Alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays, and can detect HiF-1 Alpha from human, mouse, or rat. The HiF-1 DNA Binding Activity Assay takes only 4.5 hours to complete compared to a standard 3 day electromobility shift (EMSA) method.