OxiSelect™ Glutathione Reductase Assay Kit

Oxidative Stress/ Damage->Antioxidant Assays->Glutathione Reductase Assay

Upon receipt, store the NADPH at -80ºC. Prepare single use aliquots and avoid multiple freeze/thaw cycles. Store the remaining kit components at 4ºC.

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Cell Biolabs’ OxiSelect™ Glutathione Reductase Assay Kit is a   Glutathione reductase activity is defined as 1 unit of enzyme reducing 1 µmole oxidized glutathione (GSSG) per minute at pH 7.6 and 25ºC.  The kit employs a simple enzymatic recycling reaction for glutathione quantification where the reduction of a chromagen is correlated to glutathione reductase enzymatic activity.  The kit has a detection sensitivity limit of approximately 0.6 mU/mL.  Each kit provides sufficient reagents to perform up to 100 assays, including standard curve and unknown samples.  

Colorimetric