OxiSelect™ Nε-(carboxyethyl) lysine (CEL) Competitive ELISA Kit

2 parts: 1 RT / 1 Blue Ice

Provides rapid detection and quantitation of CEL protein adducts
Perform up to 96 assays, including standard curve and unknown protein samples
Will not cross react with CML protein adducts

Oxidative Stress/Damage

First, a CEL conjugate is coated on the ELISA plate.  The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate.  After a brief incubation, the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody.  The content of CEL protein adducts in unknown samples is determined by comparison with the predetermined CEL-BSA standard curve.  Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in the OxiSelect™ CEL ELISA Kit will not cross react with CML protein adducts.

41116158