Recombinant Protein purified from E. coli

An anti-MuLV p30 monoclonal coating antibody Is adsorbed onto a microtiter plate. MuLV core antigen (p30) present In the sample or standard binds to the antibodies adsorbed on the plate; an anti-MuLV p30 polyclonal antibody Is added and binds to the antigen captured by the first antibody. Following Incubation and wash steps, a HRP-conjugated secondary antibody Is added and binds to the anti-MuLV p30 polyclonal. Unbound HRP-conjugated secondary antibody Is removed during the wash steps, and substrate solution reactive with HRP Is added to the wells. A colored product Is formed In proportion to the amount of MuLV core antigen present In the sample. The reaction Is terminated by addition of acid and absorbance Is measured at 450 nm. A standard curve Is prepared from recombinant MuLV p30 core antigen and sample concentration Is then determined.

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