293 Cell Lysate, Flag-transfected

Dry Ice

Lysate

293 cells transfected with a Flag-tagged minigene, 60kD.

Human embryonic kidney (HEK) 293 cells were originally derived from human embryonic kidney tissue and subsequently shown to be a useful cell type for the production of adenoviruses, other vial vectors, and effectively glycosylated human recombinant proteins. Such cells are therefore used for virology studies and transfected to express recombinant proteins for studies in a number of different fields.

Flag lysate is a lysate produced from 293 cells transfected with a 60kD DYKDDDDK-tagged minigene.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 10ug per lane, mini gel.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and freeze at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.