Rabbit Anti-Bovine Collagen cocktail (types 1, 3) pAb

Antibodies to calf collagen type I and III are raised in rabbits which are numerously immunized with extensively purified native collagen type I and III extracted from bovine skin into dilute acidic buffer after mild pepsin digestion. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction.The affinity purified antibody PS068 is obtained by binding to immobilized native calf collagen, mixture of type I and III (the antigens used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization.Specificity was ascertained by direct ELISA using pure calf collagen types I and III for coating microplate wells. No binding to calf serum proteins is revealed at simular dilutions of the antibody. Characteristic immunostaining pictures of frozen sections of bovine kidney and skin are produced to certify absence of crossreactivity with basement membrane collagens (types IV and V).