Mouse Anti-Human Lamin B Clone ZL-5 mAb

0.2 mg/ml

Lamins are classified into A- and B- type isoforms. Specifically, four distinct lamin isoforms have been identified: A, B1, B2, and C. Lamin A and C are alternatively spliced products of a single gene, whereas lamin B1 and B2 are encoded by distinct genes. During mitosis, lamins are phosphorylated by p34cdc2 and solubilized prior to chromosome condensation and nuclear breakdown. A direct role for lamins in chromosome condensation is suggested by the fact that lamin B binds to specific DNA sequence motifs termed matrix attachment regions (MARs). MARs mediate the interaction of chromatin with the nuclear matrix. Some of the same processes that involve mitotic chromosome condensation and nuclear envelope breakdown are also activated during apoptosis. It appears that chromosome condensation during apoptosis is accompanied by degradation of lamin protein. Lamin degradation occurs prior to DNA fragmentation and this degradation is useful as an apoptotic marker.

Recombinant human Laminin B

The antibody solution should be gently mixed before use.

Recognizes the 68 kDa Laminin B1 isoform. Reactivity with other laminin isoforms has not been detected.