Mouse Anti-Human MMP8 mAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Clon 7A11
Isotype IgG2
Applikationen WB, IHC
Menge 100 ug
Host Mouse
ArtNr CS-CMM117
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western blot: (1:400-1, 000). Immunohistochemistry (Paraffin): (1:50-200) The optimal concentration should be determined by the user for each specific application.
Description
Mouse Anti-Human MMP8 monoclonal antibody. The antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human recombinant human MMP-8). Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF alpha and GM-CSF. MMP8 is capable of cleaving types I, II and III triplehelical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP8 consists of several domains: a prodomain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexinlike domain. MMP8 is heavily glycosylated.
Formulation
Lyophilized from a sterile filtered solution in PBS
Specificity
Recognizes Human MMP8
Immunogen
Recombinant Human MMP8
Purification
Protein G affinity chromatography
Reconstitution
Centrifuge vial prior to opening. Add 0.5 mL sterile distilled water.
Storage and Stability
Lyophilized samples are stable for at least 2 years from date of receipt when stored at <=-20C. Reconstituted antibody can be aliquotted and stored frozen at <=-20C for at least 6 months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Cross-Reactivity
No cross-reactivity with other human MMPs
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 659,93 €
Preis: 659,93 €
lieferbar

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Arbeitsanleitung

CS-CMM117.pdf