Mouse Anti-Human E-Selectin/CD62E Clone 9E7 mAb

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Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Clon 9E7
Isotype IgG2
Applikationen WB
Menge 100 ug
Host Mouse
ArtNr CS-CMS100
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Applications
Western Blot, Immunohistochemistry
Description
Mouse Anti-Human E-Selectin/CD62E monoclonal antibody clone 9E7 E-Selectin is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat domains that contain 6 conserved cysteine residues. E-Selectin is a part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis.
Formulation
Lyophilized from a 0.2 um sterile filtered solution in PBS
Specificity
Recognizes Human E-Selectin/CD62E
Immunogen
Recombinant Human E-Selectin/CD62E
Purification
Protein G affinity chromatography
Reconstitution
Centrifuge vial prior to opening. Add 500 ul sterile PBS to the vial to fully solubilize the antibody to a concentration of 200 ug/ml.
Storage and Stability
Lyophilized antibody is stable for 2 years from date of receipt when stored at -80C. Reconstituted antibody can be aliquoted and stored frozen at -20C to -80C for at least six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Cross-Reactivity
No cross-reactivity to ICAM and VCAM
Application Notes
For Western Blot, use a working dilution of 1:500-2000.For Immunohistochemistry (Paraffin sections), use a working dilution of 1:50-200.The optimal concentration should be determined by the user for each specific application.

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Menge: 100 ug
Lieferbar: In stock
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