Vergleich

Anti-Human Tissue Inhibitor Metallopeptidase 3 mAb

ArtNr CS-CMT152
Hersteller Cell Sciences
Menge 100 ug
Kategorie
Typ Antibody Monoclonal
Applikationen WB, IHC
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Similar products SFD, HSMRK222, K222, K222TA2, MIG-5 protein, tissue inhibitor of metalloproteinase 3
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Storage Conditions
Lyophilized antibody is stable for 2 years at -20C to -80C. Reconstituted antibody is stable for six months in working aliquots at -20C to -80C. Avoid repeated freeze-thaw cycles.
Application Notes
For Western Blot, use a working dilution of 1:100-1000.For Immunohistochemistry (Paraffin), use a working dilution of 1:50-200.The optimal concentration should be determined by the user for each specific application.
Cross-Reactivity
This antibody recognizes human TIMP-3 in Western blot. No cross-reactivity with human TIMP-1, TIMP-2, and TIMP-4
Description
Mouse Anti-Human TIMP-3 monoclonal antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified recombinant human tissue inhibitor of metalloproteinases-3 (TIMP-3). The IgG1 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography. TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.
Formulation
Lyophilized from a 0.2 um sterile filtered solution in PBS
Immunogen
Recombinant Human TIMP-3
Purification
Protein G affinity chromatography
Reconstitution
Centrifuge vial prior to opening. Add 500 ul sterile PBS to the vial to fully solubilize the antibody to a concentration of 200 ug/ml.
Specificity
Recognizes Human TIMP-3

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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