ArtNr |
CS-CMT152 |
Hersteller |
Cell Sciences
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody Monoclonal |
Applikationen |
WB, IHC |
Specific against |
Human (Homo sapiens) |
Host |
Mouse |
Isotype |
IgG1 |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Similar products |
SFD, HSMRK222, K222, K222TA2, MIG-5 protein, tissue inhibitor of metalloproteinase 3 |
Lieferbar |
|
Storage Conditions |
Lyophilized antibody is stable for 2 years at -20C to -80C. Reconstituted antibody is stable for six months in working aliquots at -20C to -80C. Avoid repeated freeze-thaw cycles. |
Application Notes |
For Western Blot, use a working dilution of 1:100-1000.For Immunohistochemistry (Paraffin), use a working dilution of 1:50-200.The optimal concentration should be determined by the user for each specific application. |
Cross-Reactivity |
This antibody recognizes human TIMP-3 in Western blot. No cross-reactivity with human TIMP-1, TIMP-2, and TIMP-4 |
Description |
Mouse Anti-Human TIMP-3 monoclonal antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified recombinant human tissue inhibitor of metalloproteinases-3 (TIMP-3). The IgG1 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography. TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14. |
Formulation |
Lyophilized from a 0.2 um sterile filtered solution in PBS |
Immunogen |
Recombinant Human TIMP-3 |
Purification |
Protein G affinity chromatography |
Reconstitution |
Centrifuge vial prior to opening. Add 500 ul sterile PBS to the vial to fully solubilize the antibody to a concentration of 200 ug/ml. |
Specificity |
Recognizes Human TIMP-3 |
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