Mouse Anti-Human VEGF Clone 3 (6D3) mAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Clon 3(6D3)
Isotype IgG1
Applikationen WB, ELISA
Menge 200 ug
Host Mouse
ArtNr CS-CMV015
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western Blot: working dilution of 2-10 ug/ml ELISA: working dilution of 1-15 ug/ml Neutralization
Description
Human Vascular Endothelial Growth Factor VEGF165, a 23 kDa protein consisting of 165 amino acid residues, is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor, in vivo . Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). In addition to its action as a mitogen it is a potent vascular permeability factor (VPF), in vivo . VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bioavailability is very different. There is good evidence that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.
Formulation
Lyophilized from a solution in water + sodium phosphate + Trehalose + Polysorbart 20
Specificity
Human VEGFA
Immunogen
Recombinant human VEGF165
Purification
Protein G chromatography
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage and Stability
The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8C. Frozen aliquots are stable for at least 6 months when stored at -20C. Avoid repeated freeze/thaw cycles.
Application Notes
The optimal concentration should be determined by the user for each specific application.
Menge: 200 ug
Lieferbar: In stock
Listenpreis: 494,49 €
Preis: 494,49 €
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Arbeitsanleitung

CS-CMV015.pdf