Rabbit Anti-Human EGF Affinity Purified pAb

Western Blot: To detect rIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rIL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. ELISA: Indirect: To detect rIL-10 by indirect ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant rIL-10. Sandwich: To detect rIL-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant rIL-10.

Lyophilized from a solution in PBS, pH 7.2

Recombinant human EGF

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.