Antibodies

Rabbit Anti-Human EGF Affinity Purified pAb

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Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Applikationen WB, ELISA
Menge 100 ug
Host Rabbit
ArtNr CS-CPE101
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Applications
Western Blot: To detect rIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rIL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. ELISA: Indirect: To detect rIL-10 by indirect ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant rIL-10. Sandwich: To detect rIL-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant rIL-10.
Description
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human EGF. Anti-human EGF specific antibody was purified by affinity chromatography employing immobilized human EGF matrix
Formulation
Lyophilized from a solution in PBS, pH 7.2
Specificity
Human EGF
Immunogen
Recombinant human EGF
Purification
Affinity chromatography
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage and Stability
The lyophilized antibody is stable for at least 2 years from date of receipt at -20C. The reconstituted antibody is stable for at least 2 weeks at 2-4C. Frozen aliquots are stable for at least 6 months when stored at -20C. Avoid repeated freeze-thaw cycles.

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Menge: 100 ug
Lieferbar: In stock
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