Antibodies

Rabbit Anti-Human Hsp27 Affinity Purfied pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Isotype IgG
Applikationen WB, ICC, ELISA
Menge 100 ug
Host Rabbit
ArtNr CS-CPH101
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western Blot ELISA Immunoprecipitation Immunocytochemistry
Description
Rabbit Anti-Human HSPB2 polyclonal antibody. Human HSPB2, mouse Hspb1 and alphabeta-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. HSPB2 is believed to exist mainly as oligomers of as many as 8-40 HSPB2 protein monomers in cells. Data suggests that the large oligomers of HSPB2 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and HSP70-dependent refolding can occur. HSPB2 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing HSPB2. Data from studies using wild-type HSPB2 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of HSPB2. Recent findings indicate that HSPB2 is also a negative regulator of cytochrome c-dependent activation of procaspase-3.
Formulation
Liquid in PBS, pH 7.2 + 50% glycerol + 1% BSA + 0.02% thimerosal. Precaution: Thimerosal is a poisonous and hazardous substance which should be handled by trained staff only.
Specificity
Detects a 27 kDa protein, corresponding to the apparent MW = HSPB2 on SDS-PAGE immunoblots.
Purification
Affinity Purified
Storage and Stability
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
Concentration
0.2 mg/ml
Cross-Reactivity
Reacts with human, monkey, dog (weakly), and pig.
Application Notes
For Western Blot, use a working dilution of 1-2 ug/ml.For ELISA, use a working dilution of 1 ug/ml.For Immunoprecipitation, use a working dilution of 5-10 ug/ml.For Immunocytochemistry, use a working dilution of 10-15 ug/ml.The optimal concentration should be determined by the user for each specific application.
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 780,52 €
Preis: 780,52 €
lieferbar

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