Antibodies

Rabbit Anti-Human Hsp70 pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Isotype IgG
Applikationen WB
Menge 100 ug
Host Rabbit
ArtNr CS-CPH104
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
For Western Blot, use a working dilution of 1-2 ug/ml. The optimal concentration should be determined by the user for each specific application.
Description
Rabbit Anti-Human HSPA4 polyclonal antibody. HSPA4, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but are localized within different intracellular compartments. These include: a) the constitutive HSPA8 (or cognate) present within the cytosol/nucleus, b) the highly stress-inducible HSPA4 present within the cytosol/ nucleus/nucleolus, c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum, and d) the glucose regulated 75 kDa protein present within the mitochondria. HSPA4 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of HSPA4 using an antibody specific for the inducible form is useful in ascertaining whether a stress response has occurred in the cell. It should be noted, however, that there is a constitutive level of HSPA4 expression in some cell types such as primates, e.g. human and monkey. Nevertheless increased expression of HSPA4 is observed in such cells following stress.
Formulation
Liquid in PBS, pH 7.2 + 30% glycerol + 1% BSA + 0.02% thimerosal. Precaution: Thimerosal is a poisonous and hazardous substance which should be handled by trained staff only.
Specificity
Recognizes the inducible form of HSPA4 (Hsp72).
Immunogen
Synthetic peptide surrounding amino acid 559 of human HSPA4
Storage and Stability
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
Cross-Reactivity
Reacts with human, mouse, rat, cow, chicken, rabbit, monkey, hamster, dog, pig. Does not cross-react with the constitutive HSPA8 (Hsc73).
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 599,26 €
Preis: 599,26 €
lieferbar

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Arbeitsanleitung

CS-CPH104.pdf