Antibodies

Rabbit Anti-Human IGF-II pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Applikationen WB, ELISA
Menge 100 ug
Host Rabbit
ArtNr CS-CPI152
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Applications
Neutralization ELISA Western Blot
Description
Rabbit Anti-Human IGF2 Affinity Purified pAb
Formulation
A sterile filtered antibody solution (1 mg/ml) was lyophilized from 0.5X PBS, pH 7.2.
Specificity
Human
Immunogen
Recombinant Human IGF-II
Purification
Purified by affinity chromatography employing immobilized human IGF2 matrix.
Reconstitution
Centrifuge vial prior to opening . Add sterile distilled water to a concentration of 0.1-1.0 mg/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.
Storage and Stability
Antibody is stable at room temperature for at least one month and for greater than a year when kept at -20C. The antibody is stable for at least six weeks at 2-4C when reconstituted.
Application Notes
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of human IGF2 (10 ng/ml), a concentration of 3.1 4.6 mg/ml of this antibody is required. ELISA:To detect human IGF2 by direct ELISA (using 100 il/well antibody solution) a concentration of at least 0.5 ig/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant human IGF2. Western Blot:To detect human IGF2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ig/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IGF2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 756,25 €
Preis: 756,25 €
lieferbar

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