Rabbit Anti-Human p53 Affinity Purified pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Isotype IgG
Applikationen WB
Menge 100 ug
Host Rabbit
ArtNr CS-CPP138
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western Blot
Description
p53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.
Formulation
100 ug (0.5 mg/ml) affinity purified rabbit anti-p53 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol + 0.5% BSA + 5 mM EDTA + 0.01% thiomersal. Precaution: Thiomersal is a poisonous and hazardous substance which should be handled by trained staff only.
Specificity
p53
Immunogen
Synthetic peptide corresponding to residue surrounding amino acid 375 of human p53
Purification
Affinity purified
Storage and Stability
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
Concentration
0.5 mg/ml
Cross-Reactivity
Human, mouse, rat, dog, and rabbit
Application Notes
For Western Blot, use a working dilution of 0.5-4 ug/ml.Blocking Peptide (Cat. No. CPP138BP) is available separately.The optimal concentration should be determined by the user for each specific application. Other applications have not been determined.
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 659,19 €
Preis: 659,19 €
lieferbar

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