Antibodies

Rabbit Anti-Human PARP1 Affinity Purified pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Isotype IgG
Applikationen WB
Menge 100 ug
Host Rabbit
ArtNr CS-CPP143
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western blot Immnohistochemistry
Description
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases, in vitro, and is one of the main cleavage targets of caspase-3, in vivo . The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis, in vitro .
Formulation
100 ug (0.2 mg/ml) affinity purified rabbit polyclonal anti-cleaved PARP antibody in phosphate-buffered saline (PBS) containing 50% glycerol + 1% BSA, and 0.02% thiomersal. Precaution: Thiomersal is a poisonous and hazardous substance which should be handled by trained staff only.
Specificity
The antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP. Camptothecin treated Jurkat cell lysate can be used as a positive control.
Immunogen
Synthetic peptide correspond to the N-terminal residues of the catalytic domain of human PARP
Purification
Affinity purified
Storage and Stability
Store at -20C. For long term storage, aliquot and freeze at -80C. Avoid repeated freeze/defrost cycles.
Concentration
0.2 mg/ml
Cross-Reactivity
Human
Application Notes
Western blot analysis (0.5-4 ug/ml) and immunocytochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP. Camptothecin treated Jurkat cell lysate can be used as a positive control.
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 1.235,48 €
Preis: 1.235,48 €
lieferbar

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