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Rabbit Anti-Human PARP1 Affinity Purified pAb

Rabbit Anti-Human PARP1 Affinity Purified pAb

Hersteller	Cell Sciences
Kategorie	Oncology Antibodies By Cell Type Carcinoma Hematology Lymphoma Melanoma Sarcoma Cell Biology Cytoskeleton Western Blot Primary Antibodies small GTPases Arf Subfamily
Typ	Antibody Polyclonal
Specific against	Human
Isotype	IgG
Applikationen	WB
Menge	100 ug
Host	Rabbit
ArtNr	CS-CPP143
Targets	ARFRP1, PARP1
eClass 6.1	32160702
eClass 9.0	32160702
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Application Notes	Western blot analysis (0.5-4 ug/ml) and immunocytochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP. Camptothecin treated Jurkat cell lysate can be used as a positive control.
Concentration	0.2 mg/ml
Cross-Reactivity	Human
Description	PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases, in vitro, and is one of the main cleavage targets of caspase-3, in vivo . The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis, in vitro .
Formulation	100 ug (0.2 mg/ml) affinity purified rabbit polyclonal anti-cleaved PARP antibody in phosphate-buffered saline (PBS) containing 50% glycerol + 1% BSA, and 0.02% thiomersal. Precaution: Thiomersal is a poisonous and hazardous substance which should be handled by trained staff only.
Immunogen	Synthetic peptide correspond to the N-terminal residues of the catalytic domain of human PARP
Purification	Affinity purified
Storage and Stability	Store at -20C. For long term storage, aliquot and freeze at -80C. Avoid repeated freeze/defrost cycles.
Specificity	The antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP. Camptothecin treated Jurkat cell lysate can be used as a positive control.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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CS-CPP143-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 100 ug

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Lieferung vsl. bis 16.05.2024

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