Rabbit Anti-Human VEGFR-2/KDR pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Applikationen WB, ELISA
Menge 100 ug
Host Rabbit
ArtNr CS-CPV103A
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Applications
ELISA: use at 5-15 ug/ml. Western blot: use at 1-5 ug/ml. Immunoprecipitation: use 1-2 ug/mg protein lysate. The optimal concentration should be determined by the user for each specific application.
Description
Rabbit Anti-human VEGFR2/KDR polyclonal antibody Soluble KDR domains 1-7 is produced as a non-chimeric protein in a monomeric form. The soluble receptor protein consists of all 7 extracellular domains, which contain all the information necessary for high affinity ligand binding. The receptor monomers have a mass of approximately 116 kDa. Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signaling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR-2 leading to their proliferation. The binding of VEGF165 to VEGFR-2 is dependent on heparin.
Formulation
Lyophilized from PBS
Specificity
Human VEGFR2/KDR
Immunogen
Recombinant human soluble extracellular domain of KDR protein (D1-7)
Purification
Protein A chromatography
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water to the vial to fully solubilize the antibody to a concentration of 0.1-1.0 mg/ml.
Storage and Stability
Lyophilized antibody is best stored desiccated below 0C. Reconstituted antibody is stable for 2 weeks at 2-4C or in working aliquots at -20C for at least 6 months. Avoid repeated freeze-thaw cycles.
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 398,34 €
Preis: 398,34 €
lieferbar

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