Rabbit Anti-Human VEGFR3/FLT4 Affinity Purified pAb

Western Blot: When used at a concentration of 0.5-1 ug/ml, in conjunction with compatible secondary reagents, the detection limit is approximately 5 ng/lane under reducing conditions, depending on the visualization method used. ELISA: When used at a concentration of 0.5-1.5 ug/ml, in conjunction with compatible secondary reagents, allows the detection of 0.25- 0.5 ng/well. Immunoprecipitation: Use 1-5 ug IgG per 1 ml lysate or reaction volume. The optimal concentration should be determined by the user for each specific application.

Lyophilized from PBS, pH 7.4, without preservative

Recombinant human soluble VEGFR3 protein (110 kDa)

Centrifuge vial prior to opening . Add sterile PBS or distilled water to the vial to fully solubilize the antibody to a concentration > 0.5 mg/ml.

Shows moderate cross-reactivity with VEGFR2/KDR and a weak cross-reactivity with VEGFR1/FLT1.