Mouse Anti-Human PLAU Clone PGM2001 mAb

The antibody binds to human high molecular weight single-chain urokinase type plasminogen activator (HMW-scuPA, 54 kDa), to high molecular weight two-chain urokinase type plasminogen activator (HMW-tcuPA, 52 kDa) and to low molecular weight single-chain urokinase type plasminogen activator (LMW-scuPA, 33 kDa) with high affinity (Kd= 0.1 nM) and does inhibit the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is not inhibited. The antibody is highly specific for urokinase, no cross reactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human plasma or plasma proteins do not interfere with its binding to urokinase. Immunoglobulin type:Murine IgG1Use:The antibody can be used for detection of urokinase (RIA, ELISA), affinity purification and for studies on the structural and kinetic parameters of the enzyme. The antibody can be used in immunoblotting (Western, dot-blot) and immunohistochemistry on frozen sections.