Mouse Anti-Human MUC1 Clone VU-4H5 mAb

VU-4H5 reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 aminoacids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is upregulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of mAb VU4H5 is APDTR as established with “epitope fingerprinting”. Source: A Balb/c mouse was immunized with a MUC1 60mer tandem repeat NH2-(VTSAPDTRPAPGSTAPPAHG)3-COOH conjugated to bovine serum albumin. Splenocytes were fused with SP2/0 mouse myeloma cells. Stability of the hybridomas was established by subcloning four times.

100 ug/ml.