Rat Anti-Human ABCC5 Clone M5I-1 mAb

M5I-1 reacts with an internal epitope of MRP5, a 190-200 kD transmembrane protein that is closely related to the multidrug resistance protein MRP. M5I-1 was raised against a bacterial fusion protein of MRP5, containing amino acids 82-168 of the protein. M5I-1 does not cross-react with the human MDR1, MRP1, MRP2 or MRP3 gene products. Immunoglobulin type:Rat IgG2a Use: M5I-1 has potential value for detection of MRP5-mediated drug-resistance in human tumor samples. Immunocytochemistry: use 1:20-50 dil. on acetone fixed cytospin preparations. For immunohistochemistry: M5I-1 (use 1:20) on acetone fixed frozen sections can be followed by incubation with rabbit anti-rat Ig (1:25, Dako) and a monoclonal rat APAAP complex (1:50, Dako). Alternatively, after incubation with M5I-1(use 1:20) and washing, slides can be incubated with biotinylated rabbit anti-rat Ig (1:100, Jackson, West Grove) and streptavidin conjugated to horseradish peroxidase (1:500, Zymed, San Francisco, CA). Flow cytometry: optimal conditions still to be defined. Western blotting: use 1:20-50 dil. and anti-rat-HRP.