Rat Anti-Mouse ABCG2/BCRP Clone BXP-9 mAb

Specificity: The BXP-9 Mab was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the mouse bcrp protein corresponding to amino acids 221-394. BXP-9 reacts with an internal epitope of bcrp, a 70 kD transmembrane half-transporter which is involved in Multidrug resistance. BXP-9 does not react with the human BCRP molecule. Product Form: 1 ml vials containing antibody in serumfree culture supernatant, with 0.7% BSA and 0.1% Sodium azide. Concentration approx. 250 ug immunoglobulin/ml. Applications: Immunocytochemistry: working dilution of 1:20 ñ 1:50 on acetone fixed cytospin preparations. Immunohistochemistry: BXP-9 (dilution of 1:20) on acetone fixed frozen sections can be followed by incubation with biotin-labeled rabbit anti-rat IgG (1:100, DAKO) and HRP-labeled streptavidin (1: 500, DAKO). Western Blotting: working dilution of 1:20 ñ 1:50 and HRP-anti-rat-IgG. Flow Cytometry: optimal conditions still to be defined.