Rabbit Anti-Human ICAM1/CD54 Biotin pAb

Antibodies

For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end a small fresh skin biopsy can be incubated for 4 hours with TNF-a (1 ng/ml), IL-1 (100 U/ml) or LPS (1 ig/ml) in tissue culture medium at 37C.

0.5 ml (100 ug/ml) 0.2 um filtered biotinylated antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin. Precaution: Sodium azide is a poisonous and hazardous substance which should be handled by trained staff only.