GOAT Anti-Human Collagen-5 pAb

Antibodies to human collagen type V are raised in goats which are numerously immunized with extensively purified native collagen type V extracted from human placenta into dilute acidic buffer after mild pepsin digestion. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized total human serum proteins in order to remove non-specific antibodies. Next, the antisera fraction is absorbed with immunobilized collagen types I, II, III, and IV to remove cross-reactive antibodies to antigenic determinants common for various collagen types. The affinity purified antibody PS064 is obtained by binding to immobilized native human collagen type V (the antigen used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization.Specificity was ascertained by competition ELISA. Complete inhibition was found if the antibody was preincubated with collagen type V. Inhibition by other types of collagens is observed only at 20-50 times higher concentration. No inhibition was found with fribronectin, fibrinogen and laminin. Characteristic immunostaining pictures of frozen sections of human kidney, liver, skin and heart are produced to certify batch quality.