Rabbit Anti-Human ACE pAb

Human Angiotensin (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu) synthesed and purified to 98% is linked to bovine serum albumin by means of carbodiimide in random orientation. This conjugate is used to immunize rabbit. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized human albumin and immmunoglobuline fractions in order to remove non-specific antibodies.Specificity of the antisera is confirmed by its ability to bind and precipitate labeled angiotensin I and to be adsorbed by angiotensin. Native angiotensin competes with such binding, while human whole plasma or plasma proteins do not. The antiserum also reacts with angiotensin II.