Rabbit Anti-Human CASP9 (IN2) Affinity Purified pAb

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (3). Caspase-9 is also activated by granzyme B and CPP32 (1, 2). Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFa, TRAIL, anti-CD-95, FADD, and TRADD (4). Caspase-9 is expressed in a variety of human tissues (1, 2). This polyclonal antibody can be used for detection of caspase-9 by Western blot at 1:1000 to 1:2000 dilution. Whole cell lysate from HeLa cells can be used as positive control and a 46 kDa band should be detected. It has no cross response to other members in caspase family. This antibody is for research use only.