Rabbit Anti-Human CASP8AP2 (CT) Affinity Purified pAb

A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1, 2). FLASH is widely expressed. This polyclonal antibody can be used for detection of FLASH by Western blot at 0.25 to 0.5 ug/ml. HeLa cell lysate can be used as positive control and an approximate 220 kDa band can be detected. For research use only.