Rabbit Anti-Human TACE/ADAM17 (CT) Affinity Purified pAb

Tumor-necrosis factor-a is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-a is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-a. TNF-a converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately (1-3). TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFa, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP) (4, 5). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association (6). This polyclonal antibody can be used for detection of TACE by Western blot at 1:500 to 1:2000 dilution. HeLa or Jurkat whole cell lysate can be used as positive control and 80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE. It is human, mouse, and rat reactive. For research use only.