Rabbit Anti-Human IRAK3 Affinity Purified pAb

Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (1, 2), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M (3). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-kB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS. This polyclonal antibody can be used for detection of IRAK-M by Western blot at 0.5 to 1 ug/ml. Mouse spleen or rat kidney tissue lysate can be used as positive control and a 68 kDa major band can be detected. It is human, mouse, and rat reactive. Anti-IRAK-M has no cross response to IRAK or IRAK2. For research use only.