Rat Anti-Mouse EPHA3 Clone 7C13 mAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Mouse
Clon 7C13
Isotype IgG2
Applikationen WB
Menge 100 ug
Host Rat
ArtNr CS-CME116
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Applications
Western Blot: (1:100-1000)
Description
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with recombinant mouse EphA3 extracellular domain. IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
Formulation
The antibody was purified from tissue culture supernatant by affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS).
Specificity
This antibody was selected for its ability to detect mouse EphA3 in western blots. In this format, this antibody does not cross-react with recombinant mouse EphA2, EphA6, EphA4, EphA7, EphA8 and EphB2, EphB3, EphB6.
Immunogen
Mouse EphA3 extracellular domain
Purification
Protein A/G affinity chromatography
Reconstitution
Centrifuge vial prior to opening. Reconstitute the antibody with 500 ul sterile water and the final concentration is 200ug/ml. Reconstituted antibody can be aliquoted and stored frozen at < -20 for at least for six months without detectable loss of activity.
Storage and Stability
Store lyophilized antibody at -80C. Reconstituted antibody is stable for 6 months in working aliquots at -80C. Avoid repeated freeze-thaw cycles.
Application Notes
For Western Blot, use a working dilution of 1:100-1:1, 000.The optimal concentration should be determined by the user for each specific application.
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 786,58 €
Preis: 786,58 €
lieferbar

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