Antibodies

Rat Anti-Mouse IL-1R2 Clone 7D7 mAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Mouse
Clon 7D7
Isotype IgG
Applikationen WB, IHC
Menge 100 ug
Host Rat
ArtNr CS-CMI249
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western Blot, Immunohistochemistry (paraffin)
Description
Rat Anti-Mouse IL1R2 monoclonal antibody Clone 7D7. IL1 receptor type II (IL1R-II) is a 65 kD member of the immunoglobulin superfamily also known as IL1 receptor (IL1R) type II. The IL1R family is composed of three proteins: IL1R type I, IL1R type II, and the widely expressed IL1R AcP. Both IL1R type I and type II bind IL1a and IL1beta, although with differing affinities. The high affinity complex consists of IL1R type I and IL1R AcP, whereas IL1 binds to IL1R type II with low affinity. The IL1 receptor antagonist (IL1RA) can bind to both IL1R type I and II. IL1R type II is expressed at low levels on B-cells, monocytes/macrophages, neutrophils, basal epithelia of the skin, and T-cells. Binding of IL1 to the high affinity IL1 receptor mediates thymocyte and T-cell activation, fibroblast proliferation, and induction of acute phase protein production. Binding of IL1 to CDw121b appears to be dispensable for IL1 signaling and may negatively regulate IL1 responses acting as a "decoy" receptor.
Formulation
Lyophilized from a 0.2 um sterile filtered solution in PBS
Specificity
Recognizes mouse IL1R2
Immunogen
Recombinant extracellular domain of mouse IL1R2
Purification
Protein A/G affinity chromatography of tissue culture supernatant IgG fraction.
Reconstitution
Centrifuge vial prior to opening. Reconstitute with 200 ul sterile PBS (final concentration 500ug/ml).
Storage and Stability
Lyophilized antibody is stable for 2 years at -80C. Reconstituted antibody is stable for six months in working aliquots at -20 to -80C. Avoid repeated freeze-thaw cycles.
Application Notes
For Western Blot, use a working dilution of 1:1000.For immunohistochemistry, use a working dilution of 1:50 to 1:100. The optimal concentration should be determined by the user for each specific application.

Arbeitsanleitung

CS-CMI249.pdf

Menge: 100 ug
Lieferbar: In stock
Listenpreis: 725,92 €
Preis: 725,92 €
lieferbar

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