Armenian Hamster Anti-Mouse TNF-alpha Clone TN3-19.12 LE/NA mAb

TNF-alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-alpha. Monomeric mouse TNF-alpha is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-alpha forms multimeric complexes, stable trimers are most common in solution. A 26 kD membrane form of TNF-alpha has also been described. TNF-alpha binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines, in vitro , hemorraghic necrosis of tumors, in vivo , increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The TN3-19.12 antibody reacts with mouse, rat, and rabbit tumor-necrosis factor-alpha (TNF-alpha) proteins. The TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-alpha.

E. coli, -expressed, recombinant mouse TNF-alpha

1.0 mg/ml