Rabbit Anti-Mouse FGF-9 Affinity Purified pAb

Antibodies

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mouse FGF-9 (1.50 ng/ml), a concentration of 0.025-0.06 ug/ml of this antibody is required.ELISA: To detect mouse FGF-9 by direct ELISA (using 100 ul/well antibody solution) a concentration of at least 0.5 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant mouse FGF-9.Western Blot: To detect mouse FGF-9 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents, the detection limit for recombinant mouse FGF-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. The optimal concentration should be determined by the user for each specific application.

Lyophilized from a concentrated (1.0 mg/ml) sterile filtered solution containing 0.5X PBS, pH 7.2.

Affinity chromatography

Mouse FGF-9