|
Hersteller |
Cell Sciences |
|
Kategorie |
|
|
Typ |
Antibody |
|
Specific against |
Rat |
|
Isotype |
IgG |
|
Applikationen |
WB |
|
Menge |
100 ug |
|
Host |
Rabbit |
|
ArtNr |
CS-CPH102 |
|
eClass 6.1 |
32160702 |
|
eClass 9.0 |
32160702 |
|
Lieferbar |
 |
| Applications |
Western Blot |
| Description |
HSPB1 exhibits a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and HSP70-dependent refolding can occur. HSPB1 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing HSPB1. Data from studies using wild-type HSPB1 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of HSPB1. Recent findings indicate that HSPB1 is also a negative regulator of cytochrome c-dependent activation of procaspase-3. |
| Formulation |
100 ug (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol + 0.5% BSA + 0.01% thiomersal. Precaution: Thiomersal is a poisonous and hazardous substance which should be handled by trained staff only. |
| Specificity |
The antibody detects 27 kDa rat Hsp27. Rat kidney tissue lysate can be used as a positive control. |
| Immunogen |
Synthetic peptide surrounding amino acid 193 or rat HSPB1 |
| Purification |
Biospecific affinity chromatography |
| Storage and Stability |
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles. |
| Concentration |
0.5 mg/ml |
| Cross-Reactivity |
Rat |
| Application Notes |
For Western Blot, use a working dilution of 0.5-4 ug/ml.Rat kidney tissue lysate can be used as a positive control.Blocking Peptide (Cat. No. CPH102BP) is available separately.The optimal concentration should be determined by the user for each specific application. |
