Mouse IL-10 Matched Antibody Pair for ELISPOT

Capture antibody (2 vials) Product:Rat monoclonal antibody to mouse interleukin-10 (IL-10) Clone: QSM-1 Production: In vitro, using serum free medium. Purification: Protein A affinity chromatography. Contents: Adjusted amount for coating of five 96-well ELISPOT plates. Buffer: Prior to lyophilization: 0.25 ml PBS + 125 mM trehalose. Application: For use as coating antibody in an ELISPOT system, reconstitute the contents of the vial in 0.25 ml distilled water and dilute 100 times in PBS. The total amount is sufficient for 5 ELISPOT plates (480 determinations, 50 uI/well). Detector antibody (2 vials) Product:Biotinylated rat monoclonal antibody to mouse interleukin-10 (IL-10) Clone: QSM10 Production: In vitro, using serum free medium. Labeling: With N-hydroxysuccinimidobiotin Contents:Adjusted amount for five 96-well ELISPOT plates. Buffer: Prior to lyophilization: 0.5 ml PBS + 1% BSA + 125 mM trehalose. Application: For use as detector antibody in an ELISPOT system, reconstitute the contents of the vial in 0.5 ml distilled water and dilute 100 times in PBS + 1% BSA. The total amount is sufficient for 5 ELISPOT plates (480 determinations, 100 uI/well). General Specificity:Validated for detecting mouse IL-10. Sterility: Membrane filtered (0.2 um). Stability: The lyophilized products are stable for more than one year at 4C. After reconstitution, the antibodies are stable for several months at 4C (if kept sterile) or for minimal one year at -20C.