Human PAI1/tPA Complex ELISA Kit

Plasminogen Activator Inhibitor 1 (PAI1) is the principal inhibitor of Tissue Plasminogen Activator (tPA) and Urokinase (uPA), the activators of Plasminogen and hence fibrinolysis. It is a serine protease inhibitor (serpin) protein (SERPINE1). PAI1 is mainly produced by the endothelium, but is also secreted by other tissue types, such as adipose tissue. Tissue Plasminogen Activator (tPA/PLAT) is a secreted serine protease which converts the proenzyme Plasminogen to Plasmin, a fibrinolytic enzyme. tPA is synthesized as a single chain which is cleaved by Plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. It is also implicated in events related to cell invasion/migration. Plasminogen Activator Inhibitor type 1 (PAI1/SERPINE1) is involved in the regulation of the blood fibrinolytic system and forms a 1:1 covalent complex with tPA and Urokinase Plasminogen Activator (uPA/PLAU).