Mouse UPA ELISA Kit

All kit components must be stored at 2-4C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -80C for later use. DO NOT freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 2-4C. The kit should not be used after the expiration date.

Mouse uPA activity assay is intended for the quantitative determination of active plasminogen activator in mouse plasma. Urokinase plasminogen activator is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration Functionally active uPA will bind to the biotinylated human PAI-1 coated on the microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. Inactive or complexed enzyme will not be detected. After appropriate washing steps, monoclonal mouse anti-mouse uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase (HRP). TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.