Mouse Fibrinogen ELISA Kit

Range: 3.125 - 800 ng/ml. Incubation Time: 2.0 HoursSample Type: Plasma and Serum Sample Size: 100 ulThis Mouse Fibrinogen Antigen ELISA assay is intended for the quantitative determination of total Fibrinogen antigen in mouse plasma and serum. Mouse Fibrinogen will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-mouse Fibrinogen primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse Fibrinogen. Color development is proportional to the concentration of Fibrinogen in the samples.