Recombinant Bovine BTC

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Bovine
Menge 20 ug
Host E.coli
ArtNr CS-ARU020
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Bovine Betacellulin (BTC), a member of the EGF family, is an 80 amino acid heparin-binding protein. Like other members of the EGF family, it is synthesized as a transmembrane precursor. The soluble cytokine, containing one EGF structural motif, is released by proteolytic cleavage. BTC can bind to the EGF receptor (ErbB-1) which then dimerizes with another member of the ErbB receptor family to signal through the tyrosine kinase pathway. BTC is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells and is the only member of the EGF family to stimulate insulin secretion in pancreatic b cells.
Formulation
Lyophilized from 0.05 M acetic acid + 0.05% (v/v) trifluoroacetic acid and stored under dry nitrogen at a slight vacuum (ca.25 kPa)
Reconstitution
See Protocol #1500
Storage and Stability
Lyophilized protein is stable for 2 years at 2-4C. Avoid repeated freeze-thaw cycles .
Source
E. coli
Molecular Weight
8.995 kDa
Purity
> 95% bovine betacellulin, comprising an equal mixture of [Gly1]BTC and Des(1-4)BTC
Protein Accession No
NP_776321
Endotoxin Level
< 0.1 ng/ug of BTC
Biological Activity
Determined by cell growth bioassay in Balb/c 3T3 fibroblasts. The ED50 is < 10 ng/ml.
Menge: 20 ug
Lieferbar: In stock
Listenpreis: 384,19 €
Rabatt: -10,0%
Preis: 345,77 €
Sie sparen: 38,42 €
lieferbar

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Arbeitsanleitung

CS-ARU020.pdf