Recombinant Human IGF-1*di-Biotin

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 50 ug
ArtNr CS-AQU050
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Biotinylated human insulin-like Growth Factor-I (biotinyl-IGF-I) is made by reacting human IGF-I with Sulfo-NHS-Biotin reagent to label the primary amines of the IGF-I molecule. A mixture of biotinylated forms are produced, the major species being di-biotinyl-IGF-I. Biotinyl IGF-I has been prepared with a spacer to minimize steric hindrance during subsequent avidin binding.
Formulation
Lyophilized white powder, dried from Acetonitrile / 0.1% TFA under dry nitrogen at a slight vacuum (ca.25 kPa)
Reconstitution
Centrifuge vial prior to opening. Dissolve in 10 mM HCl at a concentration of at least 0.1 mg/ml.
Storage and Stability
Lyophilized product is stable for at least 2 years at 2-4C. After reconstitution, store in working aliquots below -20C. Inclusion of a carrier protein (RIA grade BSA) is recommended where possible. Avoid repeated freeze-thaw cycles.
GeneID
3479
Source
High purity IGF1 was used as the source material.
Purity
Di-biotinyl IGF-I was confirmed as the major species by Mass Spectrometry.
Biological Activity
Western ligand blot to bovine IGFBP-2

Arbeitsanleitung

CS-AQU050.pdf

Menge: 50 ug
Lieferbar: In stock
Listenpreis: 580,33 €
Preis: 580,33 €
lieferbar

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Arne Pelz

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