Recombinant Human IFN-gamma

Biomolecules

After receipt, this product should be kept at -70aC or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes in order to avoid repeated freeze-thaw cycles. Dilute only in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA).

Interferon was titrated with the use of a cytopathic effect inhibition assay. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for human interferon gamma (hIFN-Ã) provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations, " in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14 23]. Unit of activity measured on VERO cells with vesicular stomatitis virus (VSV).