|
Hersteller |
Cell Sciences |
|
Kategorie |
|
|
Typ |
Proteins |
|
Specific against |
Human |
|
Menge |
20 ug |
|
Host |
E.coli |
|
ArtNr |
CS-CRA001B |
|
eClass 6.1 |
34160400 |
|
eClass 9.0 |
42020190 |
|
Lieferbar |
 |
| Description |
Artemin is a novel member of the glial cell line-derived neurotrophic factor (GNDF) ligand family. Current evidence suggests that Artemin signals through the receptor complex, GFRalpha3-RET, to influence neuron survival, in vitro, and, in vivo . Recombinant human Artemin is a non-glycosylated, disulfide-linked homodimer, containing two 113 amino acid chains. |
| Formulation |
Recombinant Artemin is lyophilized with 10 mM sodium citrate pH 4.5 and 25 mM NaCl. |
| Reconstitution |
Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. |
| Storage and Stability |
Lyophilized product is very stable at -20C. Reconstituted material should be aliquoted and frozen at -20C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles. |
| GeneID |
9048 |
| Source |
E. coli |
| Molecular Weight |
24.2 kDa |
| Purity |
98.0% as determined by Reducing and Non-reducing SDS-PAGE, HPLC |
| Protein Accession No |
Q5T4W7 |
| Endotoxin Level |
Measured by kinetic LAL analysis and is typically <= 1 EU/ug protein. |
| Biological Activity |
The activity is determined by the dose-dependant proliferation of the SH-SY5Y cell line and is typically 4-8 ng/mL. The activity can also be determined by its ability to promote survival and neurite outgrowth. |
| AA Sequence |
AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG |
